Mouse anti Human CD11a conjugated with Biotin
| Code | Size | Price |
|---|
| EXA-11A3 | 100 Tests | £221.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: OFYS
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2 to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Product Form:
Biotin
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD11a Biotin
UniProt:
P20701
References
1. Protein kinase C-mediated regulation of the expression of CD14 and CD11/CD18 in U937 cells. Pedrinaci S; Ruiz-Cabello F; Gomez O; Collado A; Garr Int J Cancer 1990 Feb 1;45(2):294-8
2. Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils. Hartnell A; Moqbel R; Walsh GM; Bradley B; Kay AB Immunology 1990 Fe;69(2):264-70
3. Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Arnaout MA Immunol Rev 1990 Ap;114 p145-80
4. Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells. Scand J Immunol 1990 Au;32(2):137-47
5. Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. van Dijken PJ; Ghayur T; Mauch P; Down J; Burakoff SJ; Ferrara JL Transplantation 1990 Ma;49(5):882-6
6. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Patarroyo M; Prieto J; Rincon J; Timonen T; Lundberg C; Lindbom L; Asjo B; Gahmberg CG Immunol Rev 1990 Ap;114 p67-108
7. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins Anderson DC and Springer TA, Ann Rev Med 1987;38:175
2. Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils. Hartnell A; Moqbel R; Walsh GM; Bradley B; Kay AB Immunology 1990 Fe;69(2):264-70
3. Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Arnaout MA Immunol Rev 1990 Ap;114 p145-80
4. Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells. Scand J Immunol 1990 Au;32(2):137-47
5. Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. van Dijken PJ; Ghayur T; Mauch P; Down J; Burakoff SJ; Ferrara JL Transplantation 1990 Ma;49(5):882-6
6. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Patarroyo M; Prieto J; Rincon J; Timonen T; Lundberg C; Lindbom L; Asjo B; Gahmberg CG Immunol Rev 1990 Ap;114 p67-108
7. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins Anderson DC and Springer TA, Ann Rev Med 1987;38:175