Mouse anti Human CD45RA

Product Code: EXA-045R1

Code	Size	Price

EXA-045R1

100 ug

£196.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG1

Antibody Clonality: Monoclonal

Antibody Clone: BHKG-2

Regulatory Status: RUO

Target Species: Human

Application: Flow Cytometry

Shipping:

Room Temperature

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:

Add 10 ?l of MAB / 100 ?l of 10^6 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 ?l of MAB / 100 ?l of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Background:

CD45RA recognizes Mr. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

See vial for concentration

Field of Interest:

Flow Cytometry

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Flow Cytometry Staining

Immunogen:

Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Protein A/G Chromatography

Synonyms:

CD45RA

UniProt:

P08575

References

1. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634

2. CD45 isoform expression on human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM

3. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM

4. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS

5. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H

6. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace DL