Mouse anti Human CD56 conjugated with PerCP
| Code | Size | Price |
|---|
| EXA-0565 | 100 Tests | £296.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: C5.9
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8? C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. Exalpha?s CD56, clone C5.9 has been recognized as the superior CD56 antibody for Flow Cytometry applications because of it?s specific and intense staining. In fact, our CD56 is bright enough to be used in FITC! Now this antibody is proven to work in formalin fixed paraffin embedded section. Available concentrated, pre-diluted in dropper bottles, or conjugated to several flurochromes for manual use or automated applications.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Product provided titered for use in flow cytometry when used as indicated below.
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD56=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.
Label:
PerCP
Product:
PerCp conjugated monoclonal antibody C5.9, rovided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.
Synonyms:
CD56, NCAM1, Neural Cell Adhesion Molecule
UniProt:
P13591