Mouse anti Human CD71
| Code | Size | Price |
|---|
| EXA-0711 | 100 ug | £196.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: BGX.24
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC. Antibodies should be stored at 4-8oC. Monoclonal antibodies should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.
Background:
CD71 is specific for the human transferrin receptor, MW 190 kdaltons (kDa), 90 kDa reduced. CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Flow Cytometry
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Flow Cytometry Staining
Immunogen:
Derived from the hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with blast cells from pokeweed mitogen-stimulated peripheral blood cells.
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Derived from the hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with blast cells from pokeweed mitogen-stimulated peripheral blood cells.
Synonyms:
CD71
UniProt:
P02786
References
1. Newman R, Schneider C, Sutherland R, Vodinelich L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982;1:397.
2. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W, Do?rken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press;1989:455-460.
3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1984;312:162-163.
4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980;152:1430.
5. Phillips JH, Le AM. Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J Exp Med. 1984;159:993-1008.
6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.
2. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W, Do?rken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press;1989:455-460.
3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1984;312:162-163.
4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980;152:1430.
5. Phillips JH, Le AM. Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J Exp Med. 1984;159:993-1008.
6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.