Mouse anti Human CD35 conjugated with FITC

Product Code: EXA-0352

Code	Size	Price

EXA-0352

100 Tests

£221.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG1

Antibody Clonality: Monoclonal

Antibody Clone: AR2

Regulatory Status: RUO

Target Species: Human

Application: Flow Cytometry

Shipping:

Room Temperature

Storage:

Product should be stored at 4-8°C. DO NOT FREEZE

Further Information

Applications Description:

PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Background:

Complement Receptor-1 (CR-1), CD35 complex is four different allotypes (C,A,B,D) C is 160 kDa; A is 190 kDa; B is 220 kDa and D is 250 kDa. It is involved in the processing of immune complexes, promoting of binding and phagocytosis of C3b complexes and inhibition of complement activation. CR-1 is on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukemias, follicular dendritic reticulum cells and is negative on basophils.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

Titered for flow cytometry

Field of Interest:

Flow Cytometry

Formulation:

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Functional Analysis:

Flow Cytometry Staining

Immunogen:

CD35=Derived from the hybridization od mouse SP2/0 cells with spleen cells of mice immunized with purified CR1

Product Form:

FITC

Product Stability:

Reagents are stable for the period shown on the vial label when stored properly

Purification Method:

Protein A/G Chromatography

Synonyms:

CD35 FITC

UniProt:

P17927

References

1. Differential effects of the stimulation of complement receptors CR1 (CD35) and CR2 (CD21) on cell proliferation and intracellular Ca2+ mobilization of chronic lymphocytic leukemia B cells. Hivroz C; Fischer E; Kazatchkine MD; Grillot-Courvalin C J Immunol 1991 Mar 1;146(6):1766-72

2. The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. Moulds JM; Nickells MW; Moulds JJ; Brown MC; Atkinson JP J Exp Med 1991 May ;173(5):1159-63

3. Deduction from Wilms' tumour that glomerular podocytes produce the basement membrane material bearing Goodpasture determinants. Droz D; Diebold N; Jan D; Jaubert F J Pathol 1990 De;162(4):323-7

4. Purification and characterization of IgG immunoconglutinins from patients with systemic lupus erythematosus: implications for a regulatory function. Nilsson B; Ekdahl KN; Svarvare M; Bjel Clin Exp Immunol 1990 No;82(2):262-7

5. Granulocyte-macrophage colony-stimulating factor increases synthesis and expression of CR1 and CR3 by human peripheral blood neutrophils. Neuman E; Huleatt JW; Jack RM J Immunol 1990 Nov 1;145(10):3325-32

6. Expression of CR1 (CD35) mRNA in podocytes from adult and fetal human kidneys. Appay MD; Kazatchkine MD; Levi-Strauss M; Hinglais N; Bariety J Kidney Int 1990 Au;38(2):289-93

7. Complement receptor type 1 (C3b/C4b receptor; CD35) and complement receptor type 2 (C3d/Epstein-Barr virus receptor; CD21). Fearon DT; Ahearn JM Curr Top Microbiol Immunol 1990;153 p83-98