Mouse anti Human CD15

Product Code: EXA-0151

Code	Size	Price

EXA-0151

100 ug

£258.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgM

Antibody Clonality: Monoclonal

Antibody Clone: ARE

Regulatory Status: RUO

Target Species: Human

Application: Flow Cytometry

Shipping:

Room Temperature

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:

PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Background:

Identification of CD15 that recognizes a human mylelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.1. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils. It is present at low density on circulating monocytes. In lymphoid tissue CD15 reacts with Reed-Sternberg cells of Hodgkins disease and with granulocytes. However, CD15 reacts with only a few tissue macrophages and does not react with dendritic cells.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

See vial for concentration

Field of Interest:

Flow Cytometry

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Flow Cytometry Staining

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Protein A/G Chromatography

Synonyms:

CD15

UniProt:

P22083

References

1. Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Do?rken B, Gilks WR, et al, eds. LeucocyteTyping IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989;800-805.

2. Hanjan SNS, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopath. 1982;23:172.

3. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984;82:29.

4. Pinkus GS, Thomas P, Said JW. Leu-M1?A marker for Reed Sternberg cells in Hodgkin's Disease: An immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.