Mouse anti Human CD16

Product Code: EXA-0161

Code	Size	Price

EXA-0161

100 ug

£196.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG1

Antibody Clonality: Monoclonal

Antibody Clone: J5511

Regulatory Status: RUO

Target Species: Human

Application: Flow Cytometry

Shipping:

Room Temperature

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:

PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2 to 8? C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacture?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Background:

Identification of human NK cell antigen expressing the 50 -70 kDa M.W. surface antigen associated with the IgG Fc receptor III on NK cells and Neutrophils. CD16 is expressed on approximately 15% of peripheral blood lymphocytes and is present on all resting NK cells. CD16 may be expressed on CD3 T cells from certain individuals.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

See vial for concentration

Field of Interest:

Flow Cytometry

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Flow Cytometry Staining

Immunogen:

CD16=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with NK lymphocytes

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Synonyms:

CD16

UniProt:

Q9ULV2

References

1. CD16 on human gamma delta T lymphocytes: expression, function, and specificity for mouse IgG isotypes. Braakman E; van de Winkel JG; van Krimpen BA; Jansze M; Bolhuis RL Cell Immunol 1992 Aug;143(1):97-107

2. Fc gamma RIII activation is different in CD16+ cytotoxic T lymphocytes and natural killer cells. Uciechowski P; Gessner JE; Schindler R; Schmidt RE Eur J Immunol 1992 Jun;22(6):1635-8

3. Involvement of a metalloprotease in spontaneous and phorbol ester-induced release of natural killer cell-associated Fc gamma RIII (CD16-II). Harrison D; Phillips JH; Lanier LL J Immunol 1991 Nov 15;147(10):3459-65

4. CD16- CD56+ natural killer cells after bone marrow transplantation. Jacobs R; Stoll M; Stratmann G; Leo R; Link H; Schmidt RE Blood 1992 Jun 15;79(12):3239-44

5. Natural killer function in flow cytometry: identification of human lymphoid subsets able to bind to the NK sensitive target K562. Vitale M; Zamai L; Neri LM; Manzoli L; Facchini A; Papa S Cytometry 1991;12(8):717-22

6. In vitro responsiveness to interleukins and theophylline of CD16+, CD56- natural killer cells in a patient with chronic granular lymphocyte disorder. Bayle C; Vitte-Mony I; Lang P; Pico J; Hercend T; Bertoglio J Leukemia 1992 May;6(5):470-6

7. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain. Wirthmueller U; Kurosaki T; Murakami MS; Ravetch JV J Exp Med 1992 May 1;175(5):1381-90