Mouse anti Human CD18 conjugated with Biotin
| Code | Size | Price |
|---|
| EXA-0183 | 100 Tests | £221.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3EB
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
LFA-1 beta is a leukocyte adhesion receptor to (ICAM's) intercellular adhesion molecules. LFA-1 beta is on virtually all peripheral blood lymphocytes, 95,000 M.W. surface antigen.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Label:
Biotin
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Product Form:
Biotin
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD18* Biotin
UniProt:
P05107
References
1. CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the alpha chain of fibrinogen. Loike, J.D., Sodeik, B., Cao, L., Leucona, S., Weitz, J.I., Detmers, P.A., Wright, S.D., Silverstein, S.C.. Proc. Natl. Acad. Sci. USA, 1991 Feb; 88(3):1044-8.
2. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Dustin, M.L. and Springer, T.A.. Nature. 1989:341:619.
3. Coupling of the adhesive receptor CD11/CD18 to functional enhancement of effector macrophage tissue factor response. Fan, S.T. and Edgington, T.S.. J. Clin. Invest. 1991 Ja:87(1):50-7.
4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med.1987;38:175.
5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15.
6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin -triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA, 1990 De;87(23):9472-6.
2. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Dustin, M.L. and Springer, T.A.. Nature. 1989:341:619.
3. Coupling of the adhesive receptor CD11/CD18 to functional enhancement of effector macrophage tissue factor response. Fan, S.T. and Edgington, T.S.. J. Clin. Invest. 1991 Ja:87(1):50-7.
4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med.1987;38:175.
5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15.
6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin -triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA, 1990 De;87(23):9472-6.