Mouse anti Human CD23 conjugated with PE
| Code | Size | Price |
|---|
| EXA-0234 | 100 Tests | £258.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: LVNC-4
Regulatory Status: RUO
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/106 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
CD23 antigen is an integral membrane glycoprotein, Mr. 45 kdaltons The CD23 antigen is present on approximately all pre-B lymphocytes, plasma cells CD25 positive Ki-1 cells and HLA-DR. CD23 antigen is also expressed on Lymphoma, T Cell Lymphomas and B-CLLs. CD23 antigen is present on Hodgkin?s and Reed Sternberg cells).
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Product Form:
RPE
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
UniProt:
P06734
References
1. CD23: a multi-lineage cell activation molecule with a split personality. Int J Clin Lab Res 1992; 22(2):73-80Malavasi F; Funaro A; Alessio M; DeMonte LB; Ausiello CM; Dianzani U; Lanza F; Magrini E; Momo M; Roggero S
2. Eleven lymphoid phenotypic markers in HIV infection: selective changes induced by zidovudine treatment. J Acquir Immune Defic Syndr 1992;5(9):890-7Bass HZ; Hardy WD; Mitsuyasu RT; Wang YX; Cumberland W; Fahey JL
3. Three-color cytofluorometric analysis of CD8 cell subsets in HIV-1 infection. J Acquir Immune Defic Syndr 1991;4(12):1227-32 Prince HE; Jensen ER
2. Eleven lymphoid phenotypic markers in HIV infection: selective changes induced by zidovudine treatment. J Acquir Immune Defic Syndr 1992;5(9):890-7Bass HZ; Hardy WD; Mitsuyasu RT; Wang YX; Cumberland W; Fahey JL
3. Three-color cytofluorometric analysis of CD8 cell subsets in HIV-1 infection. J Acquir Immune Defic Syndr 1991;4(12):1227-32 Prince HE; Jensen ER