Mouse anti Human CD61 conjugated with PE

PBMC: Add 5 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2 to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 5 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

CD61 recognizes an Mr 110-kdalton (kDa) protein, also known as gpIIIa, the common ?subunit (integrin ?3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. The CD61 antigen is also found on endothelial cells, megakaryocytes, on some myeloid, erythroid and T-lymphoid leukemic cell lines.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Titered for flow cytometry

Flow Cytometry

Provided as solution in phosphate buffered saline, pH 7.3, with 0.2% BSA and 0.08% sodium azide

Flow Cytometry Staining

CD61=Derived from the hybridization of mouse X63.Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with purified platelet membrane glycoproteins.

RPE

Reagents are stable for the period shown on the vial label when stored properly

Protein A/G Chromatography

P05106