Rabbit anti Human Sphingosine Kinase 2 Long Form

Antibody can be used for Western blotting (1-5 ?g/ml) and ELISA. Optimal concentration should be evaluated by serial dilutions.

The long form of sphingosine kinase 2 (Sphk2-L) is a species-specific isoform of the Sphk2 enzyme expressed in human but not in mouse. It is an N-terminal extended form of Sphk2, extended by 36 amino acids. It functions as expected, catalyzing the formation of sphingosine 1-phosphate from sphingosine, however it appears to have a decreased ability to inhibit DNA synthesis as compared to Sphk2-S (short form of Sphk2). Under normal cellular conditions, Sphk2-L does not appear to inhibit DNA synthesis, but under serum deprivation, SphK2-L translocates to the nucleus and accumlates. This accumlation may be involved in the cessation of cell proliferation or apoptosis depending on the cell type.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Lipid Mediators

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting, ELISA

Synthetic peptide derived from the N-terminal extension of the Sphk2-L protein.

HEK293 cells

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Sphk2-L, N-terminal-extended Sphingosine Kinase 2

AF245447