Mouse anti Human CD57

Nordic MuBio
Product Code: EXA-X2781M
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-X2781M200 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: NK-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Antibody can be used for Western blotting (1-10 ?g/ml), Immunoprecipitation (1-2 ?g per 100-500 ?g of total protein), Immunofluorescence (2-20??g/ml), Immunohistochemistry (2-20 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced from mice immunized with human peripheral blood mononuclear cells.
Positive Control:
Human lymph node, Tonsil
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
HNK1, GLCATP, NK-1, HNK-1, LEU7, NK1, 151290, B3GAT1, Q9P2W7, GlcUAT-P, CD57, GlcUATP, GlcAT-P, 27087, NK 1, HNK 1, GlcAT P, GlcUAT P
UniProt:
Q9P2W7

References

Abo, T., et al. J. Immuno., 127, 1024 (1984)