Rabbit anti Human Chromogranin A
| Code | Size | Price |
|---|
| EXA-X2789P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for immunohistochemistry (1-3 ?g/ml) and Western blotting (1-2 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Chromogranin A is the major protein co-stored and co-released with catecholamines from secretory vesicles in adrenal medulla and postganglionic sympathetic axons. It is required for formation of catecholamine secretory vesicles in chromaffin cells and its expression may be sufficient even to induce a regulated secretory system in non-secretory cells. It is also a pro-hormone that gives rise to biologically active peptides such as the dysglycemic peptide pancreastatin, the antimicrobial peptide chromacin, the vasodilator vasostatin, and catestatin that acts to inhibit catecholamine release.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from human Chromogranin A protein
Positive Control:
Human small bowel tissue
Product Form:
Affinity Purified
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
SP-1, Pituitary Secretory Protein I
UniProt:
P10645
References
1. Konecki, D.S., et al. ?The primary structure of human chromogranin A and pancreastatin? J. Biol. Chem., 262, 17026-17030 (1987)
2. Pasqua, T., et al. ?Full-length human chromogranin-A cardioactivity: myocardial, coronary, and stimulus-induced processing evidence in normotensive and hypertensive male rat hearts.? Endocrinology, 154, 3353-3365 (2013)
3. Chen, Y., et al. ?Naturally occurring genetic variants in human chromogranin A (CHGA) associated with hypertension as well as hypertensive renal disease.? Cell Mol. Neurobiol., 30, 1395-1400 (2010)
4. Mahapatra, N.R., et al. ?Hypertension from targeted ablation of chromogranin A can be rescued by the human ortholog.? J. Clin. Invest., 115, 1942-1952 (2005)
5. Mahata, S.K., et al. ?Novel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.? J. Clin. Invest., 100, 1623-1633 (1997).
2. Pasqua, T., et al. ?Full-length human chromogranin-A cardioactivity: myocardial, coronary, and stimulus-induced processing evidence in normotensive and hypertensive male rat hearts.? Endocrinology, 154, 3353-3365 (2013)
3. Chen, Y., et al. ?Naturally occurring genetic variants in human chromogranin A (CHGA) associated with hypertension as well as hypertensive renal disease.? Cell Mol. Neurobiol., 30, 1395-1400 (2010)
4. Mahapatra, N.R., et al. ?Hypertension from targeted ablation of chromogranin A can be rescued by the human ortholog.? J. Clin. Invest., 115, 1942-1952 (2005)
5. Mahata, S.K., et al. ?Novel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.? J. Clin. Invest., 100, 1623-1633 (1997).