Rabbit anti Human Sphingomyelin Phosphodiesterase 3
| Code | Size | Price |
|---|
| EXA-X2360P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (1-5 ?g/ml starting dilution). Optimal concentration should be evaluated by serial dilutions.
Background:
Sphingomyelin phosphodiesterase 3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these two cellular events independently. It also hydrolyzes sphingosylphosphocholine. Sphingomyelin phosphodiesterase 3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It likely acts as a regulator of postnatal development and participates in bone and dentin mineralization. It is activated by unsaturated fatty acids and phosphatidylserine. it is predominantly expressed in the brain.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from human sphingomyelin phosphodiesterase 3
Positive Control:
Predominantly expressed in the brain.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
nSMase2; Neutral sphingomyelinase 2; EC 3.1.4.12
UniProt:
Q53YV1 (Human)
References
1. Hofmann, K., et al. Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase.; Proc. Natl. Acad. Sci. U.S.A. 97:5895-5900(2000).
2. Miura, Y., et al. Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases.; FEBS Lett. 557:288-292(2004).
3. Marchesini, N., et al. Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells.; J. Biol. Chem. 279:25101-25111(2004).
2. Miura, Y., et al. Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases.; FEBS Lett. 557:288-292(2004).
3. Marchesini, N., et al. Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells.; J. Biol. Chem. 279:25101-25111(2004).