Mouse anti Human Enolase
| Code | Size | Price |
|---|
| EXA-X2070M | 200 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NSE-P1
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (1-2 ?g/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, Enolase, Enolase and Enolase. Enolase expression has been detected on most tissues, whereas Enolase is expressed predominantly in muscle tissue and Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.
Positive Control:
Serum, brain, pancreas, liver, skeletal muscle and endocrine tumors. Expression stronger in tumor versus normal tissue.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
ENO2; Enolase 2, (gamma, neuronal); 2-Phospho-D-Glycerate Hydrolase
UniProt:
P09104
References
1. Kang, J.L., et al. (1996). Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ. J. Pathol. 178(2):161-165.
2. Koch, M., et al. (2007). Plasma S100beta and NSE levels and progression in multiple sclerosis. J. Neurol. Sci. 252(2):154-158.
3. Oertel, M., et al. (2006). S-100B and NSE: markers of initial impact of subarachnoid haemorrhage and their relation to vasospasm and outcome. J. Clin. Neurosci. 13(8):834-840.
2. Koch, M., et al. (2007). Plasma S100beta and NSE levels and progression in multiple sclerosis. J. Neurol. Sci. 252(2):154-158.
3. Oertel, M., et al. (2006). S-100B and NSE: markers of initial impact of subarachnoid haemorrhage and their relation to vasospasm and outcome. J. Clin. Neurosci. 13(8):834-840.