Mouse anti Human Heterogeneous Nuclear Ribonucleoprotein K

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Nordic MuBio
Product Code: EXA-X2068M
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-X2068M200 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: F45 P9 C7
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Antibody can be used for Western blotting (1-2 ?g/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken
Positive Control:
Colorectal cancer tissue, Calu6 and HT29 cell lysates
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
hnRNP-K; CSBP; TUNP; dC-Stretch Binding Protein
UniProt:
P61978

References

1. Wei, C.C., et al. (2006). Heterogeneous nuclear ribonucleoprotein K modulates angiotensinogen gene expression in kidney cells. J. Biol. Chem. 281(35):25344-25355

2. Dzwonek, A., et al. (2006). The diverse involvement of heterogeneous nuclear ribonucleoprotein K in mitochondrial response to insulin. FEBS Lett. 580(7):1839-1845

3. Carpenter, B., et al. (2006). Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer. Br. J. Cancer. 95(7):921-927