Mouse anti FITC (Fluorescein isothiocyanate)
| Code | Size | Price |
|---|
| EXA-X1542M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: F4/1
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for Western blotting (1-2 ?g/ml), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 ?g/ml) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
Background:
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversation of fluorescein-labeled detection into immunoenzymatic assays.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Ancillary Reagents
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
Positive Control:
Antibodies labeled with FITC
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
FITC
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
References
1. Balogh P; et al. 'Hapten mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody.' J. Immunol. Methods 1994, 169, 35-40.