Mouse anti Gram Negative Bacteria DNA Adenine Methylase

Antibody can be used for Western blotting (1-5 ?g/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions.

DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. Antibody can be used for Western blotting (1-5 ?g/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions. The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Infectious Agents & Disease

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Recombinant DNA Adenine Methylase protein.

Not available

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Hybridoma obtained after immunization of mice with recombinant DNA Adenine Methylase protein.

DAM; DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase

P0AEE8