Rabbit anti Human Phosphatidic Acid Phosphatase Domain Containing 2 (PPAPDC2)
| Code | Size | Price |
|---|
| EXA-X1717P | 10 Miniblots | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ship on gel ice freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (1:400-1:800 dilution). Optimal concentration should be evaluated by serial dilutions.
Background:
Upon cell activation Presqualene diphosphate (PSDP)2, a bioactive lipid, rapidly remodels to presqualene monophosphate (PSMP). A newly identified and characterized phosphatase (phosphatidic acid phosphatase domain containing 2 (PPAPDC2)) has recent;y been shown to convert PSDP to PSMP. Also known as Phosphatidic acid phosphatase type 2 domain-containing protein 2. Unlike the related polyisoprenyl phosphate farnesyl diphosphate (FDP), PSDP has been shown not to be a substrate for type 2 lipid phosphate phosphohydrolases. PSDP phosphatase activity has been identified in activated human neutrophil (PMN) extracts. Recombinant PPAPDC2 displays diphosphate phosphatase activity with substrate preference for PSDP > FDP phosphatidic acid. PPAPDC2 activity is independent of Mg2+ and optimal at pH 7.0-8.0. PPAPDC2 mRNA has been detected in human PMN, and is widely expressed in human tissues. PPAPDC2 expressed in human PMN is first lipid phosphate phosphohydrolase identified for PSDP. The regulation of this enzyme?s activity has important implications for PMN activation in innate immunity.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Lot specific, see vial
Field of Interest:
Inflammation & Immunology
Formulation:
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.1% BSA and 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from human phosphatidic acid phosphatase type 2 domain containing 2 (PPAPDC2)
Positive Control:
Testis
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Synonyms:
Phosphatidic acid phosphatase type 2 domain containing 2, PPAPDC2, PDP, PPAC2
UniProt:
Q8IY26
References
1. 'IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF A
PRESQUALENE DIPHOSPHATE PHOSPHATASE.' Koichi Fukunaga, Makoto Arita, Minoru Takahashi, Andrew J. Morris, Michael Pfeffer and Bruce D. Levy JBC Papers in Press. Published on February 7, 2006 as Manuscript M512970200
2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., , Marra M.A.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
PRESQUALENE DIPHOSPHATE PHOSPHATASE.' Koichi Fukunaga, Makoto Arita, Minoru Takahashi, Andrew J. Morris, Michael Pfeffer and Bruce D. Levy JBC Papers in Press. Published on February 7, 2006 as Manuscript M512970200
2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., , Marra M.A.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).