Mouse anti Human Prostaglandin-E2 receptor EP2/EP3
| Code | Size | Price |
|---|
| EXA-X1522M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 3F12
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for Western blot analysis (1-5 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Prostaglandins (PG's) are produced by the metabolism of arachidonic acid. PGE-2 is one of the five physiologically significant prostanoids known. It?s wide spectrum of physiologic and pharmacologic effects in various tissues are mediated through binding to the PGE-2 receptors (EP1, EP2, EP3 & EP4). These include effects on the immune, endocrine, cardiovascular, renal and reproductive systems as well as smooth muscle. It is also one of the most abundant of the prostanoid family in the brain where it plays an important role in many neural functions, particularly in newborn babies, and as a mediator of inflammation. PGE-2 signals through a family of G-protein coupled receptors known as EP receptors. There are 4 subtypes of EP receptors, known as EP1, EP2, EP3 and EP4. EP2 receptors are 358 amino acid proteins with a short third intracellular loop. EP2 receptors stimulate adenylyl cyclase by their coupling to Gs and do not undergo PGE-2-induced internalization. EP3 receptors are 365-425 amino acid proteins. There are currently 4 known isoforms of EP3 receptors named EP3A, 3B, 3C and 3D. EP3 receptors are involved in water absorption, gastric acid secretion, uterine contraction, neurotransmitter release and the hydrolysis of fat cells (lipolysis) and also act as a mediator of neural inflammation.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Inflammation & Immunology
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human EP2 and EP3 receptor protein and mouse myeloma cells.
Positive Control:
Porcine brain lysate
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
Pan Prostaglandin E2 Receptor Subtype EP2 and EP3; Provided under license from Allergan, Inc., United States Patent Pending
UniProt:
P43116
References
1. Strong, P. et al. Prostanoid-induced inhibition of lipolysis in rat isolated adipocytes: Probable involvement of EP3 receptors. Prostaglandins 1992, 43, 559-566
2. Coleman, R.A., et al. Classification of prostanoid receptors: Properties, distribution and structure of the receptors and their subtypes. Pharmacol. Rev. 1994, 46, 205-229
3. Beiche, F., et al. Localization of cyclooxygenase-2 and prostaglandin E2 receptor EP3 in the rat lumbar spinal cord. J. Neuroimmunol. 1998, 89, 26-34
4. Bhattacharya, M., et al. Nuclear prostaglandin receptors. Gene Ther. Mol. Biol. 1999, 4, 323-338
5. Morath, R., et al. Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3 and EP4 in human kidney. J. Am. Soc. Nephrol. 1999, 10, 1851-1860
6. Nakamura, K., et al. Immunohistochemical localization of prostaglandin EP3 receptor in the rat hypothalamus. Neurosci. Lett. 1999, 260, 117-120
7. Desai, S., et al. Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration. Mol. Pharmacol. 2000, 58, 1279-1286
8. Fedyk, E.R., et al. Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells. Proc. Natl. Acad. Sci. USA 1996, 93, 10978-10983
2. Coleman, R.A., et al. Classification of prostanoid receptors: Properties, distribution and structure of the receptors and their subtypes. Pharmacol. Rev. 1994, 46, 205-229
3. Beiche, F., et al. Localization of cyclooxygenase-2 and prostaglandin E2 receptor EP3 in the rat lumbar spinal cord. J. Neuroimmunol. 1998, 89, 26-34
4. Bhattacharya, M., et al. Nuclear prostaglandin receptors. Gene Ther. Mol. Biol. 1999, 4, 323-338
5. Morath, R., et al. Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3 and EP4 in human kidney. J. Am. Soc. Nephrol. 1999, 10, 1851-1860
6. Nakamura, K., et al. Immunohistochemical localization of prostaglandin EP3 receptor in the rat hypothalamus. Neurosci. Lett. 1999, 260, 117-120
7. Desai, S., et al. Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration. Mol. Pharmacol. 2000, 58, 1279-1286
8. Fedyk, E.R., et al. Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells. Proc. Natl. Acad. Sci. USA 1996, 93, 10978-10983