Mouse anti Human Estrogen Receptor

This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 ?g/mg of protein lysate), Western blotting (1-2 ?g/ml) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions.

The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.

T47D Cells

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.

P11474