Rabbit anti Human cyclin dependent kinase 2

Antibody can be used for Western blotting (1:2,000 dilution) and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Cyclin-Dependent Kinase 2 (CDK2), also known as p33cdk2 is expressed earlier in the cell cycle than is p34cdc2. Like p34cdc2, p33cdk2 associates with cyclin A in human cells. However, kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase. This antibody recognizes only phosphorylated cdk2 protein.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cell Cycle

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Synthetic peptide derived from the phosphorylated human cdk2 protein.

CDK2 expressed in baclovirus

This antibody recognizes only phosphorylated cdk2 protein.

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Rabbits were immunized with a synthetic peptide derived from the phosphorylated human cdk2 protein.

Anti-Cyclin-Dependent Kinase-2, Phosphorylated; p33cdk2

P24941