Rabbit anti Human Histone H2A.x
| Code | Size | Price |
|---|
| EXA-X2753P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme Immunoassay (EIA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (1-5 ?g/ml) and immunohistochemistry (2-5 ?g/ml at pH 9.5). Optimal concentration should be evaluated by serial dilutions.
Background:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from human histone H2a.x protein.
Positive Control:
Human bladder, ovary, colon
Product Form:
Affinity Purified
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
H2AFX; H2AX
UniProt:
P16104
References
1. Paull, T.T., et al. ?A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage? Curr. Biol., 10, 886-895 (2000)
2. Kobayashi, J., et al. ?NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.? Curr. Biol., 12, 1846-1851 (2002)
3. Stewart, G.S., et al. ?MDC1 is a mediator of the mammalian DNA damage checkpoint.? Nature, 421, 961-966 (2003)
4. Lukas, C., et al. ?Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.? EMBO J., 23, 2674-2683 (2004)
2. Kobayashi, J., et al. ?NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.? Curr. Biol., 12, 1846-1851 (2002)
3. Stewart, G.S., et al. ?MDC1 is a mediator of the mammalian DNA damage checkpoint.? Nature, 421, 961-966 (2003)
4. Lukas, C., et al. ?Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.? EMBO J., 23, 2674-2683 (2004)