Rabbit anti Human Lysophosphatidic Acid Acetyltransferse beta
| Code | Size | Price |
|---|
| EXA-X1652P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme Immunoassay (EIA)
- Western Blot (WB)
Shipping:
Ship on dry ice freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (5-10 ?g/ml) and EIA. Optimal concentration should be evaluated by serial dilutions.
Background:
Lysophospholipid acyltransferases (LPLATs) catalyse the addition of fatty acyl moieties to the glycerol backbone of lysophospholipids . In addition to playing a crutial role in the synthesis of structural membrane components the LPLATs are also implicated in cellular signalling responses for cytokines, growth factors and other agonists. Cellular signalling through the interleukin 1 receptor in human mesangial cells and EL-4 cells proceeds by the activation of lysophosphatidic acid acyltransferase (LPAAT). Activation of LPAAT by interleukin 1 results in the generation of unsaturated phosphatidic acid species, that are crucial to the generation of diacylglycerol and interleukin 1 signalling. LPLATs are also involved in signalling for increased interleukin 2 synthesis through the T cell antigen receptor. Activation of T-cells via anti-CD3 stimulation has been shown to increase incorporation of polyunsaturated fatty acids into phosphatidylcholine via lysophosphatidylcholine acyltransferase. Activation of this enzyme is essential for the sustained activation and translocation of protein kinase C.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the LPAAT- protein
Positive Control:
Human brain lysate (Cat. No. X1633C)
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
1-AGP acyltransferase 2, 1-AGPAT 2,1-acylglycerol-3-phosphate O-acyltransferase 2, LPAAT-?, 2
UniProt:
O15120
References
[1] Eberhardt C., Gray P.W., Tjoelker L.W.; Human lysophosphatidic acid acyltransferase. cDNA cloning, expression, and localization to chromosome 9q34.3.; J. Biol. Chem. 272:20299-20305(1997).
[2] Stamps A.C., Elmore M.A., Hill M.E., Kelly K., Makda A.A., Finnen M.J.; A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases.; Biochem. J. 326:455-461(1997).
[3] West J., Tompkins C.K., Balantac N., Nudelman E., Meengs B., White T., Bursten S., Coleman J., Kumar A., Singer J.W., Leung D.W.; Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells.; DNA Cell Biol. 16:691-701(1997).
[4] Leung D.W., Tompkin C.K., West J.; Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[6] Garg A.; Acquired and inherited lipodystrophies.; N. Engl. J. Med. 350:1220-1234(2004).
[7] Agarwal A.K., Arioglu E., de Almeida S., Akkoc N., Taylor S.I., Bowcock A.M., Barnes R.I., Garg A.; AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34.; Nat. Genet. 31:21-23(2002).
[2] Stamps A.C., Elmore M.A., Hill M.E., Kelly K., Makda A.A., Finnen M.J.; A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases.; Biochem. J. 326:455-461(1997).
[3] West J., Tompkins C.K., Balantac N., Nudelman E., Meengs B., White T., Bursten S., Coleman J., Kumar A., Singer J.W., Leung D.W.; Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells.; DNA Cell Biol. 16:691-701(1997).
[4] Leung D.W., Tompkin C.K., West J.; Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[6] Garg A.; Acquired and inherited lipodystrophies.; N. Engl. J. Med. 350:1220-1234(2004).
[7] Agarwal A.K., Arioglu E., de Almeida S., Akkoc N., Taylor S.I., Bowcock A.M., Barnes R.I., Garg A.; AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34.; Nat. Genet. 31:21-23(2002).