Rabbit anti Human Sphingosine 1-Phosphate Phosphatase 2
| Code | Size | Price |
|---|
| EXA-X1637P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Not applicable
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
Ship on dry ice freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (1:400 dilution). Tissue lysate should be used at 10-50 ?g/lane. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.
Background:
Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. SPPase2 is localized to the endoplasmic reticulum. The enzymatic activity and localization of SPPase2 are similar to SPPase1. The tissue expression of SPPase2 was different from that of SPPase1. SPPase2 is an important member of the SPPase family which play a role in attenuating intracellular S1P signaling.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Whole serum
Field of Interest:
Signal Transduction
Formulation:
Provided as whole antiserum
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the sphingosine 1-phosphate phosphatase 2 protein.
Positive Control:
Human brain lysate (Cat. No. X1633C)
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
Sphingosine 1-Phosphate Phosphohydrolase
UniProt:
Q8IWX5
References
1. Mechtcheriakova D, et al. Sphingosine 1-phosphate phosphatase 2 is induced during inflammatory responses. Cell Signal. 2007 Apr;19(4):748-60. Epub 2006 Sep 30.? Links
2. Grey, A., et al. 'Osteoblastic Cells Express Phospholipid Receptors and Phosphatases and Proliferate in Response to Sphingosine 1-Phosphate.' Calcid Tissue Int. 2004
3. Pettus, B.J., et al. 'The sphingosine kinase 1/sphingosine 1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha.' FASEB J. 2003, 17, 1411-1421
4. Johnson, K.R., et al. 'Role of human sphingosine 1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine 1-phosphate levels and cell viability.' J. Biol. Chem. 2003, 278, 34541-34547
5. Cuvillier, O. 'Sphingosine in apoptosis signalling.' Biochim. Biophys. Acta 2002, 1585, 153-162
6. Ogawa, C., et al. 'Identification and characterization of a novel human sphingosine 1-phosphate phosphohydrolase, hSPP2.' J. Biol. Chem. 2003, 278, 1268-1272
7. Le Stunff, H., et al. 'Characterization of murine sphingosine 1-phosphate phosphohydrolase.' J. Biol. Chem. 2002, 277, 8920-8927
8. Birchwood, C.J., et al. 'Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation.' J. Biol. Chem. 2001, 276, 11712-11718
2. Grey, A., et al. 'Osteoblastic Cells Express Phospholipid Receptors and Phosphatases and Proliferate in Response to Sphingosine 1-Phosphate.' Calcid Tissue Int. 2004
3. Pettus, B.J., et al. 'The sphingosine kinase 1/sphingosine 1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha.' FASEB J. 2003, 17, 1411-1421
4. Johnson, K.R., et al. 'Role of human sphingosine 1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine 1-phosphate levels and cell viability.' J. Biol. Chem. 2003, 278, 34541-34547
5. Cuvillier, O. 'Sphingosine in apoptosis signalling.' Biochim. Biophys. Acta 2002, 1585, 153-162
6. Ogawa, C., et al. 'Identification and characterization of a novel human sphingosine 1-phosphate phosphohydrolase, hSPP2.' J. Biol. Chem. 2003, 278, 1268-1272
7. Le Stunff, H., et al. 'Characterization of murine sphingosine 1-phosphate phosphohydrolase.' J. Biol. Chem. 2002, 277, 8920-8927
8. Birchwood, C.J., et al. 'Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation.' J. Biol. Chem. 2001, 276, 11712-11718