Rabbit anti Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
| Code | Size | Price |
|---|
| EXA-X2722P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western Blotting (1:400 dilution) and immunohistochemistry (2-10 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the human GAPDH alpha protein.
Positive Control:
Human liver tissue, human testis
Product Form:
Affinity Purified
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
UniProt:
P04406
References
1. Nakajima, H., et al. 'GAPDH aggregate formation participates in oxidative stress-induced cell death.' J. Biol. Chem. 2009:Epub
2. Nahlik, K.W., et al. 'Modulation of GAPDH expression and cellular localization after vaccinia virus infection of human adherent monocytes.' Acta Biochim. Pol. 2003: 50(3); 667-676.
2. Nahlik, K.W., et al. 'Modulation of GAPDH expression and cellular localization after vaccinia virus infection of human adherent monocytes.' Acta Biochim. Pol. 2003: 50(3); 667-676.