Rabbit anti Human SURF1
| Code | Size | Price |
|---|
| EXA-X1562P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blot (1-5 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Human Ssu72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. The human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. Rabbit polyclonal antibodies were raised against SSU72 fusion protein. The ABs recognize a single band of ~30 Kda on immunoblots of various human cell lines but do not work well on mouse tissues.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Full length recombinant human Ssu72 protein
Positive Control:
MCF-7 and Cos-7 cells
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
Human Ssu72
UniProt:
Q15526
References
1. St-Pierre, B., et al. 'Conserved and specific functions of mammalian ssu72' Nucleic Acids Res. 2005, 33, 464-477
2. Krishnamurthy, S., et al. 'Ssu72 is an RNA polymerase II CTD phosphatase' Mol. Cell 2004, 14, 387-394
3. Steinmetz, EJ., Brow, DA. 'Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription' Mol Cell Biol 2003, 23, 6339-6349
2. Krishnamurthy, S., et al. 'Ssu72 is an RNA polymerase II CTD phosphatase' Mol. Cell 2004, 14, 387-394
3. Steinmetz, EJ., Brow, DA. 'Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription' Mol Cell Biol 2003, 23, 6339-6349