Mouse anti Human Muc1

The antibody reacts with the extracellular 12-mer GVTSAPDTRPAP epitope of the MUC-1 tandem repeat.Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues and ELISA. Optimal concentration should be evaluated by serial dilutions.

Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with live human breat cancer cells (cell line ZR-75-1) and mouse myeloma SP2 cells.

MCF-7 and T47D cells

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Episialin

P15941