Mouse anti Human p21 WAF-1
| Code | Size | Price |
|---|
| EXA-X1158M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: WA-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Room Temperature
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Detects p21 protein by Western blot at 1 to 10 ?g/ml. Detects a 21 kDa protein in MCF7 cells. Can be used with either frozen or paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: ELISA
Background:
p21, also known as wild-type p53-activated factor (WAF-1) or Cdk-interacting protein (CIP1), expression is predominantly induced by p53. This expression is usually in response to DNA damage caused by cytotoxic agents. The expression of p21 interrupts the cell cycle and prevents the replication of the cell containing genomic errors. p21 inhibits Cdk2, Cdk3, Cdk4 and Cdk6 to stop the cell cycle. Contained in the human p21 is a highly conserved cystine-rich region (amino acids 21-60) with a potential zinc finger domain as well as a second conserved region (amino acids 130-164) which contains several putative nuclear localization signals. p21 can also interact with proliferating cell nuclear antigen (PCNA), Gadd45, Cdk2 and cyclins which suggests it may coordinate DNA repair and replication in damaged cells.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cell Cycle
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human p21 protein and mouse myeloma SP2 cells.
Positive Control:
MCF7 cell lysate
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
UniProt:
P38936
References
1. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878
2. El-Deiry, W.S., et al. WAF1, a potential mediator of p53 tumor suppression. Cell 1993, 75, 817-825
3. Xiong, Y., et al. p21 is a universal inhibitor of cyclin kinases. Nature (Lond) 1993, 366, 701-704 4. Zhang, H., et al. Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol. Biol. Cell 1993,
4, 897-906
5. Kovalic, J., et al. Int. J. Oncol. 1996, 9 (suppl.), 835
6. Shen, J., et al. ?Identification and Validation of Differences in Protein Levels in Normal, Premalignant, and Malignant Lung Cells and Tissues Using High-Throughput Western Array and Immunohistochemistry.? Cancer Res., 66, 11194-11206 (2006).
2. El-Deiry, W.S., et al. WAF1, a potential mediator of p53 tumor suppression. Cell 1993, 75, 817-825
3. Xiong, Y., et al. p21 is a universal inhibitor of cyclin kinases. Nature (Lond) 1993, 366, 701-704 4. Zhang, H., et al. Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol. Biol. Cell 1993,
4, 897-906
5. Kovalic, J., et al. Int. J. Oncol. 1996, 9 (suppl.), 835
6. Shen, J., et al. ?Identification and Validation of Differences in Protein Levels in Normal, Premalignant, and Malignant Lung Cells and Tissues Using High-Throughput Western Array and Immunohistochemistry.? Cancer Res., 66, 11194-11206 (2006).