Rabbit anti Human Calcium Channel

This antibody can be used for Western blotting at 5-10 ?g/ml. Optimal concentration should be evaluated by serial dilutions.

Voltage-dependent calcium channels (VDCCs) are large (>400 kDa) heteromers which contain, minimally, three core subunits 1, 2/ , in a 1:1:1 stoichiometry1. Expression of VDCC gene products in Xenopus oocytes, or transfected cells shows that the 1 subunits contain the ion channel pore while the auxiliary 2/ and subunits confer optimal cell surface expression and channel kinetics1. Until recently, the only exception to the above paradigm was the skeletal muscle VDCC, which, in addition to the 1, 2/ , core motif, also has an additional tightly associated integral membrane glycoprotein subunit termed 1. Upon co-expression with the 1.1, 2/ 1, 1a subunits of the skeletal muscle VDCC, subunits alter the peak currents, and the kinetics of channel activation and inactivation with the overall effect being a normalisation of currents to those resembling the endogenous channel2. Together, these results suggest that subunits modulate skeletal muscle VDCCs by stabilising their conformation. The 3 subunit is specifically localized in the brain, with the 2 and 4 subunits. It shares >60% sequence homology with the 2 and 4 subunits and ~25% sequence homology with the 1 and 5 subunits.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Synthetic peptide derived from the rat calcium channel gamma3 subunit conjugated to KLH

Rat brain lysate

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Neuronal voltage-gated calcium channel gamma-3 subunit; Transmembrane AMPAR regulatory protein gamma-3; Voltage-dependent calcium channel gamma-3 subunit

O60359