Rabbit anti Human Lysophosphatidic Acid Receptor 1 CT (EDG-2)

For Western blotting, starting dilution of 5-10 ?g/ml is recommended. Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. Pierce?s SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions.

Endothelial cell differentiation gene-2 (EDG-2) receptor is a high affinity receptor for lysophosphatidic acid (LPA). EDG-2, also known as lysophospholipid receptor A1, Vzg-1, mrec1.3 or GPCR26, like all other EDG receptors, couple multiple (3 or more) types of G proteins and transduce decreases in c[AMP]i through Gi and increases in [Ca2+]i by augmenting phospholipase C through Gq/11 and beta/gamma dimers and by induction of PI3 kinase, p125 FAK, phospholipase D by activating rho through G12/13. Human EDG-2 is present in high levels on oligodendrocytes and certain human malignant T cell lines. EDG-2 receptors (with EDG-4) may play a role in protecting cardiomyocytes from apoptosis induced by hypoxia and andrenergic stimulation.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Synthetic peptide derived from the C terminal of the EDG2 receptor.

RH7777 Cells Transfected with EDG2/LPA1protein

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Endothelial Cell Differentiation Gene Receptor 2; Lysophosphatidic Acid Receptor 1

Q92633