Mouse anti Human CDC25A

Application: Western Blot, Immunoprecipitation at 1-10 ug/ml

The immunogen for the anti CDC25A was a purified recombinant CDC25A protein recognizing a 65kD protein. In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Cell Cycle

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

A purified recombinant CDC25A protein recognizing a 65kD protein

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Mice were immunized with a purified recombinant CDC25A protein of 65kD protein

Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1; MPIP1;

P30304 (Human)