Mouse anti Human Rb (Retinoblastoma)

Nordic MuBio
Product Code: EXA-N120M
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-N120M100 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: XZ104
Regulatory Status: RUO
Target Species: Human
Application: Immunoprecipitation (IP)
Shipping:
Room Temperature
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Applications: Immunoprecipitation (not recommended for Western blotting or immunohistochemistry).
Background:
The immunogen for the anti-human retinoblastoma (Rb) was a recombinant protein corresponding to amino acids 393 to 572 of the human retinoblastoma sequence. Retinoblastoma encodes a phosphorylated protein p110 and binds and regulates E2F transcription factors. Rb functions as a major cell cycle regulator by its ability to repress gene transcription by all three RNA polymerases. In its hypophosphorylated form, Rb binds to a subset of E2F containing complexes converting them to repressors preventing expression of E2F target genes. These complexes repress transcription via E2F binding elements in the promotersof cell cycle control genes, such as E2F-1, c-myc, and cdc2 genes.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cell Cycle
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Immunoprecipitation
Immunogen:
A recombinant protein corresponding to amino acids 393 to 572 of the human retinoblastoma sequence
Product Form:
Unconjugated
Product Stability:
Antibodies are stable for one year from purchase if stored frozen
Purification Method:
Protein A/G Chromatography
Synonyms:
Retinoblastoma-associated protein; pRb; pp110; p105-Rb
UniProt:
P06400 (Human)

References

1) Hu, Q.J., et. al. 'Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.' Mol. Cell Biol. 1991, 11: 5792-5799

2) Harlow, E., 'Retinoblastoma. For our eyes only.' Nature 1992, 359, 270-271

3) Dyson, N., et al. 'Adenovirus E1A makes two distinct contacts with the retinoblastoma protein.' J. Virol. 1992, 4606-4611

4) Zarkowska, T., et al., 'Monoclonal antibodies specific for underphosphorylated retinoblastoma protein identify a cell cycle regulated phosphorylation site targeted by CDKs.' Oncogene 1997, 14, 249-254

5) Lai, A., et al. 'RBP1 Recruits the mSIN3-Histone Deacetylase Complex to the Pocket of Retinoblastoma Tumor Suppressor Family Proteins Found in Limited Discrete Regions of the Nucleus at Growth Arrest.