Mouse anti Human Heat Shock Protein 27
| Code | Size | Price |
|---|
| EXA-X1264M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: G3.1
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme Immunoassay (EIA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for immunohistochemistry and immunocytochemistry on frozen and paraffin-embedded tissue sections as well as for ELISA, immunoprecipitation and Western blot. Optimal concentration should be evaluated by serial dilutions.
Background:
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosphilia HSP?s and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.
UniProt:
P04792
References
1. Adams, D.J., et al. 'Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies.' Cancer Res. 1983, 43, 4297-4301.
2. Moretti-Rojas, I., et al., 'A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.' Breast Cancer Res. Treat. 1988, 11, 155-163.
3. Hino, M., et al. 'Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.' Biochem. Biophys. Res. Commun. 2000, 271, 164-169.
4. Ciocca, D.R. and Luque, E.H. 'Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer.' Breast Cancer Res. Treat. 1991, 20, 33-42.
2. Moretti-Rojas, I., et al., 'A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.' Breast Cancer Res. Treat. 1988, 11, 155-163.
3. Hino, M., et al. 'Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.' Biochem. Biophys. Res. Commun. 2000, 271, 164-169.
4. Ciocca, D.R. and Luque, E.H. 'Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer.' Breast Cancer Res. Treat. 1991, 20, 33-42.