Rabbit anti Human DICER1
| Code | Size | Price |
|---|
| EXA-X2756P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme Immunoassay (EIA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for immunohistochemical staining (1-5 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Immunohistochemistry
Immunogen:
Synthetic peptide derived from human DICER1 protein.
Product Form:
Affinity Purified
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
Endoribonuclease Dicer; DICER; HERNA
UniProt:
Q9UPY3
References
1. Zhang, H., et al. ?Single processing center models for human Dicer and bacterial RNase III.? Cell, 118, 57-68 (2004)
2. Meister, G., et al. ?Identification of novel argonaute-associated proteins.? Curr. Biol., 15, 2149-2155 (2005)
3. Haase, A.D., et al. ?TRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.? EMBO Rep., 6, 961-967 (2005)
4. Kok, K.H., et al. ?Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.? J. Biol. Chem., 282, 17649-17657 (2007)
5. Melo, S.A., et al. ?A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.? Nat. Genet., 41, 365-370 (2009)
2. Meister, G., et al. ?Identification of novel argonaute-associated proteins.? Curr. Biol., 15, 2149-2155 (2005)
3. Haase, A.D., et al. ?TRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.? EMBO Rep., 6, 961-967 (2005)
4. Kok, K.H., et al. ?Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.? J. Biol. Chem., 282, 17649-17657 (2007)
5. Melo, S.A., et al. ?A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.? Nat. Genet., 41, 365-370 (2009)