Mouse anti Human Insulin

Antibody can be used for Western blotting (1-5 ?g/ml), immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 ?g/ml, incubate at room temp. for at least 1 hr.) and ELISA. Optimal concentration should be evaluated by serial dilutions.

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with full-length insulin protein and mouse myeloma cells.

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

P01308