Mouse anti Human Estrogen Receptor
| Code | Size | Price |
|---|
| EXA-X1266M | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: AER311
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme Immunoassay (EIA)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for staining of human and mouse frozen and paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: Immunoprecipitation, Western Blotting, ELISA.
Background:
The human estrogen receptor has a molecular weight of 67 kDa and consists of 6 functional domains (domains A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of the estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with purified estrogen receptors from calf uterus and mouse myeloma cells.
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from mice immunized with purified estrogen receptors from calf uterus and mouse myeloma cells.
UniProt:
P03372
References
1. Abbondanza, E., et al. 'Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.' Steroids 1993, 58, 4-12.
2. Santeusanio, G., et al. 'Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule.' Appl. Immunohistochem Mol Morphol. 2000, 8, 275-284.
3. Hori, M., et al. 'Determination of estrogen receptor in primary breast cancer using two different monoclonal antibodies, and correlation with its mRNA expression.' Pathol. Int. 1999, 49, 191-197.
4. Schuler, G., et al. 'Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.' Biol. Reprod. 2002, 66, 976-982.
2. Santeusanio, G., et al. 'Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule.' Appl. Immunohistochem Mol Morphol. 2000, 8, 275-284.
3. Hori, M., et al. 'Determination of estrogen receptor in primary breast cancer using two different monoclonal antibodies, and correlation with its mRNA expression.' Pathol. Int. 1999, 49, 191-197.
4. Schuler, G., et al. 'Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.' Biol. Reprod. 2002, 66, 976-982.