Mouse anti Human NPDC-1

Antibody can be used for Western blotting (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions.

The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptiona levents mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, 3mM sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide derived from human NPDC-1 protein and mouse myeloma cells.

Brain tissue lysate (mouse and rat tested, human not yet tested).

Unconjugated

Products are stable for one year from purchase when stored properly

Ion-exchange chromatography

Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide SLGSPVSSDPVHMS derived from human NPDC-1 protein (Gene ID: 56654) and mouse myeloma cells.

Monoclonal Anti-NPDC-1 (mouse IgG1 isotype) is derived from the hybridoma 16E4 produced by immunizing mice with the peptide SLGSPVSSDPVHMS (Gene ID: 56654).

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