Sheep anti Mouse Dyrk (Downs Syndrome critical region)
| Code | Size | Price |
|---|
| EXA-X1079P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Detects Dyrk by immunofluorescence at 1 to 10 ?g/ml. Detects Dyrk by Western blot at 1 ?g/ml. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Background:
Dyrk, the vertebrate of Drosophilia minibrain, is a dual-specificity kinase predominately expressed in the central nervous system. The human clone has been isolated from the Downs' syndrome critical region and it is potentially implicated in the neuropathology of the disease. Main features of the protein include an N-terminal nuclear translocation signal, a putative leucine zipper domain, a core kinase domain with some similarity to kinases involved in cell cycle regulation and a C-terminal PEST sequence. The Dyrk kinase can be phosphorylated on tyrosine residues, leading to an active kinase that can phosphorylated itself or exogenous substrates on both tyrosine and serine/threonine residues. Dyrk can also multimerize and translocate to the nucleus. Present studies on Dyrk suggest a potential role for this kinase in the exit from the cell cycle and the beginning of neuronal differentiation.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Recombinant protein corresponding to amino acids 486 to 763 of the mouse Dyrk1A protein.
Positive Control:
Expressed in a variety of embryonic and adult tissues. Expressed abundantly in neurons of the brain, spinal cord, and retina in developing embryos.
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Source:
Sheep were immunized with a recombinant protein corresponding to amino acids 486 to 763 of the mouse Dyrk1A protein.
Synonyms:
Dual Specificity (Tyrosine and serine/threonine kinase) Kinase
UniProt:
Q61214
References
1. Kentrup, H., et al., Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII. J. Biol. Chem. 1996, 271, 3488-3495.
2. Song, W.-J., et al., Isolation of human and murine homologues of the Drosophilia minibrain gene: Human homologue maps to 21q22.2 in the Down syndrome critical region. Genomics 1996, 38, 331-339.
3. Becker, W. & Joost, H.G. Structural and functional characteristics of Dyrk, a novel subfamily of protein kinases with dual specificity. Prog. Nucleic Acid Res. Mol. Biol. 1999, 62, 1-17.
2. Song, W.-J., et al., Isolation of human and murine homologues of the Drosophilia minibrain gene: Human homologue maps to 21q22.2 in the Down syndrome critical region. Genomics 1996, 38, 331-339.
3. Becker, W. & Joost, H.G. Structural and functional characteristics of Dyrk, a novel subfamily of protein kinases with dual specificity. Prog. Nucleic Acid Res. Mol. Biol. 1999, 62, 1-17.