Rabbit anti Rat Kv1.1 Potassium Channel
| Code | Size | Price |
|---|
| EXA-X1497P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for Western blotting (5-10 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
K+ channels from the Kv1 subfamily contain four alpha-subunits and the combinations (from Kv1.1-1.6) determine susceptibility to dendrotoxin (DTX) homologue. Kv1.1, a Shaker-like voltage-gated potassium channel, is strongly expressed in a variety of neurons in adult rodents, in which it appears to be involved in regulating neuronal excitability. Here we show that Kv1.1 is also expressed during embryonic development in the mouse, exhibiting two transient peaks of expression around embryonic day 9.5 (E9.5) and E14.5. Potassium channels play a critical role in limiting neuronal excitability. Mutations in certain voltage-gated potassium channels have been associated with hyperexcitable phenotypes in both humans and animals. However, only recently have mutations in potassium channel genes (i.e. KCNQ2 and KCNQ3) been discovered in a human epilepsy, benign familial neonatal convulsions.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the rat Kv1.1 potassium channel conjugated to KLH
Positive Control:
Rat brain lysate
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
Voltage-Gated Potassium Channel, Kv1.1 isoform
References
1. Baumann,A et.al Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems. EMBO J. 7 (8), 2457-2463 (1988)
2. Christie,M.J et.al. Expression of a cloned rat brain potassium channel in Xenopus oocytes
Science 244 (4901), 221-224 (1989)
3. Hatton WJ, et.al Functional and molecular expression of a voltage-dependent K(+) channel (Kv1.1) in interstitial cells of Cajal. J Physiol 2001 Jun 1 533:Pt 2 315-27(2001)
4. Wang FC et.al alpha subunit compositions of Kv1.1-containing K+ channel subtypes fractionated from rat brain using dendrotoxins. Eur J Biochem Jul 263:1 230-7 (1999)
5. Folco E, et.al A cellular model for long QT syndrome. Trapping of heteromultimeric complexes consisting of truncated Kv1.1 potassium channel polypeptides and native Kv1.4 and Kv1.5 channels in the endoplasmic reticulum J Biol Chem Oct 17 272:42 26505-10 (1997)
6. Roberds,S.L Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1798-1802 (1991)
2. Christie,M.J et.al. Expression of a cloned rat brain potassium channel in Xenopus oocytes
Science 244 (4901), 221-224 (1989)
3. Hatton WJ, et.al Functional and molecular expression of a voltage-dependent K(+) channel (Kv1.1) in interstitial cells of Cajal. J Physiol 2001 Jun 1 533:Pt 2 315-27(2001)
4. Wang FC et.al alpha subunit compositions of Kv1.1-containing K+ channel subtypes fractionated from rat brain using dendrotoxins. Eur J Biochem Jul 263:1 230-7 (1999)
5. Folco E, et.al A cellular model for long QT syndrome. Trapping of heteromultimeric complexes consisting of truncated Kv1.1 potassium channel polypeptides and native Kv1.4 and Kv1.5 channels in the endoplasmic reticulum J Biol Chem Oct 17 272:42 26505-10 (1997)
6. Roberds,S.L Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1798-1802 (1991)