Rabbit anti Rat Potassium Channel
| Code | Size | Price |
|---|
| EXA-X1504P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for Western blotting (5-10 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
The Kv3.1b potassium channel is a voltage-gated channel protein which belongs to the delayed rectifier class and to the Shaw potassium channel subfamily. Potassium channels are mainly found in plasma membranes but are not generally distributed over the cell surface. Potassium channels catalyze the rapid permeation of potassium ions while rejecting biologically abundant potential competitors such as sodium, calcium and magnesium. Ion selectivity and high through put rate of potassium channels is accomplished by precise co-ordination of dehydrated potassium by the protein and multiple ion occupancy within the permeation pathway. All potassium channels carry out the formation of a transmembrane leak specific for potassium ions. Since cells almost universally maintain cytoplasmic potassium concentrations higher than those extracellularly, the opening of a potassium channel implies a negative ongoing change in electrical voltage across the cell membrane. This may result in termination of the action potential of electrically excitable cells including nerve, muscle and pancreatic beta cells. In non?excitable cells, potassium channels play important roles in the cellular potassium recycling required for electrolyte balance effected by the renal epithelium.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the rat Kv3.1b potassium channel conjugated to KLH
Positive Control:
Rat brain lysate
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Source:
Rabbits were immunized with a synthetic peptide derived from the rat Kv3.1b potassium channel conjugated to KLH
UniProt:
P25122
References
1. Luneau,C.Jet.al. 'Alternative splicing contributes to K+ channel diversity in the mammalian central nervous system.' Proc. Natl. Acad. Sci. U.S.A. 88 (9), 3932-3936 (1991)
2. Rettig, J et.al 'Characterization of a Shaw-related potassium channel family in ratbrain' EMBO J. 11 (7), 2473-2486 (1992)
3. Hartig,W.et.al 'Perineuronal nets in the rat medial nucleus of the trapezoid body surround neurons immunoreactive for various amino acids, calcium-binding proteins and the potassium channel subunit Kv3.1b.' Brain Res. 899(1-2):123-33 (2001)
4. Osipenko,OH et.al Potential role for kv3.1b channels as oxygen sensors.Circ Res.;86(5):534-40 (2000)
2. Rettig, J et.al 'Characterization of a Shaw-related potassium channel family in ratbrain' EMBO J. 11 (7), 2473-2486 (1992)
3. Hartig,W.et.al 'Perineuronal nets in the rat medial nucleus of the trapezoid body surround neurons immunoreactive for various amino acids, calcium-binding proteins and the potassium channel subunit Kv3.1b.' Brain Res. 899(1-2):123-33 (2001)
4. Osipenko,OH et.al Potential role for kv3.1b channels as oxygen sensors.Circ Res.;86(5):534-40 (2000)