Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

Detects EDG1 receptors at concentration of 5-10 ?g/ml by Western blot using a human EDG1 receptor transfected cell line. Detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG1. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Synthetic peptide derived from the C-terminal of the EDG-1 receptor

RH7777 cells transfected with full length EDG-1 protein.

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Endothelial cell differentiation gene 1 C-terminal

P21453