Mouse anti Human Myogenin
| Code | Size | Price |
|---|
| EXA-X1415M | 100 ug | £455.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: F12B
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Gel Shift
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation (2 ?g/mg), Western blotting (1 ?g/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Positive Control:
Rhabdomyosarcoma and Wlim's tumors
Product:
X1415M, clone F12B recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Synonyms:
Myogenin, Myogenic factor 4, Myf-4
UniProt:
P15173 (Human) P12979 (Mouse) P20428 (Rat)
References
1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 19: 131-138 (1996).
2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607.
3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.
2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607.
3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.