Sheep anti Human chx 10 (Visual system homeobox 2) (CT)

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Nordic MuBio
Product Code: EXA-X1179P
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-X1179P250 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Sheep
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Detects Chx10 by Western blot at 0.5 to 1 ?g/ml. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Background:
Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Antibody raised against a recombinant protein corresponding to amino acids 264 to 361 derived from the C terminal of the human Chx10 protein conjugated to KLH.
Positive Control:
Rat or mouse retinal tissue lysate. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Product:
Antibody raised against a recombinant protein corresponding to amino acids 264 to 361 derived from the C-terminal region of the human Chx10 protein conjugated to KLH.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
Ceh-10 homeodomain-containing homolog
UniProt:
P58304

References

1. Liu, I.S., et al. Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer. Neuron 1994, 13, 377-393
2. Chen, C.M. & Cepko, C.L. Expression of Chx10 and Chx10-1 in the developing chicken retina. Mech. Dev. 2000, 90, 293-297
3. Ferda Percin, E., et al. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10. Nat. Genet. 2000, 25, 397-401
4. Nittner, D., et al. Synthetic lethality between Rb, p53 and Dicer or miR-17?92 in retinal progenitors suppresses retinoblastoma formation. Nature Cell Biology (2012), 14, 958-965
5. Kay, J.N., et al. Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate. Nature Neuroscience (2011), 14, 965-972
6. Eiraku, M., et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature (2011), 472, 51-56
7. Pierfelice, T.J., et al. Notch3 Activation Promotes Invasive Glioma Formation in a Tissue Site-Specific Manner. Cancer Res., 71, 1115-1125 (2011).