Mouse anti Human MyoD1
| Code | Size | Price |
|---|
| EXA-X1414M | 100 ug | £455.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 5.2F
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 ?g/mg of protein lysate), Western blotting (1 ?g/ml) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 ?g/ml). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.
Background:
Recognizes a protein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing?s sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Positive Control:
Rhabdomyosarcoma, SW80 cells
Product:
Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Synonyms:
Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3
UniProt:
P15172 (Human) P10085 (Mouse) Q02346 (Rat) P16075 (Chicken)
References
1. Thulasi R; et al. Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth and Differentiation, 1996 Apr, 7(4):531-41.
2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9.
3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11.
4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701.
5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9.
6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81.
2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9.
3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11.
4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701.
5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9.
6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81.