Sheep anti Human chk 1 kinase

Applications: Western Blot at 1 ? 10 ug/ml.

The immunogen for anti-human chk-1 was a protein corresponding to amino acid sequence 1-474 and having a molecular weight of 54 kD. One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

A protein corresponding to amino acid sequence 1-474 and having a molecular weight of 54 kD

Recombinant proteins conjugated to OVA, GST, His tags and other. Native chk 1 kinase.

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Sheep were immunized with a protein corresponding to amino acid sequence 1-474 of human chk-1, having a molecular weight of 54 kDa.

sheep anti human chk 1 kinase, checkpoint kinase 1

O14757