Rabbit anti Human Lipid Phosphate Phosphohydrolase 2
| Code | Size | Price |
|---|
| EXA-X1650P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Porcine (pig)
Applications:
- Enzyme Immunoassay (EIA)
- Western Blot (WB)
Shipping:
Ship on dry ice freeze upon arrival
Storage:
Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (see vial for dilution) and EIA. Optimal concentration should be evaluated by serial dilutions.
Background:
Phosphatidic acid (PA), lysophosphatidic acid, ceramide 1-phosphate (C1P), and sphingosine 1-phosphate (S1P) are lipid mediators generated by phospholipases, sphingomyelinases, and lipid kinases. The major pathway for degradation of these lipids is dephosphorylation catalyzed by members of two classes (types 1 and 2) of phosphohydrolase activities (PAPs). cDNAs encoding two type 2 PAPs, PAP-2a and -2b, have been expressed by transient transfection and shown to catalyze hydrolysis of PA, C1P, and S1P.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Lot specific, see vial
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the lipid phosphate phosphohydrolase 2 protein
Positive Control:
Human brain lysate (Cat. No. X1633C)
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
Pan Phosphatidic Acid Phosphatase 2C; Pan PPAP
UniProt:
O43688
References
[1] Leung D.W., Tompkins C.K., White T.; Molecular cloning of two alternatively spliced forms of human phosphatidic acid phosphatase cDNAs that are differentially expressed in normal and tumor cells.; DNA Cell Biol. 17:377-385(1998).
[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).
[3] Hooks S.B., Ragan S.P., Lynch K.R.; Identification of a novel human phosphatidic acid phosphatase type 2 isoform.; FEBS Lett. 427:188-192(1998).
[4] Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.; Cloning of human full-length CDSs in BD Creator(TM) system donor vector.; Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).
[3] Hooks S.B., Ragan S.P., Lynch K.R.; Identification of a novel human phosphatidic acid phosphatase type 2 isoform.; FEBS Lett. 427:188-192(1998).
[4] Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.; Cloning of human full-length CDSs in BD Creator(TM) system donor vector.; Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).