Sheep anti Mouse Bub1 beta-NT

Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis (1-10 ?g/ml). Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.

Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Recombinant protein corresponding to amino acids 10-190 of the mouse Bub1b protein.

Mitotic NIH/3T3 cells

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Mitotic checkpoint kinase MAD3L

Q9Z1S0