Sheep anti Mouse LFC
| Code | Size | Price |
|---|
| EXA-X1089P | 250 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 ?g/ml. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions.
Background:
Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
Rho guanine nucleotide exchange factor 2, ARHGEF2
UniProt:
Q92974 (HUMAN)
References
1. Glaven, J.A., et al. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells. J. Biol. Chem. 1999, 274, 2279-2285
2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381
3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395
2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381
3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395