Sheep anti Rat SSeCKS - myristylated (PKC) substrate
| Code | Size | Price |
|---|
| EXA-S125P | 250 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blot (2-5 ?g/ml), immunohistochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Background:
Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
Positive Control:
Rat aortic smooth muscle
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Source:
Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
Synonyms:
SSeCKS; A-kinase anchor protein 12; AKAP12; src-suppressed C kinase substrate
UniProt:
Q62766 (Rat)
References
1. Lin, X., et al. 'A novel src- and ras-suppressed protein kinase C substrate with cytoskeletal architecture.' J. Biol. Chem. 1996, 271, 28430-28438
2. Siegel, S.M., et al. 'SSeCKS immunolabeling in rat primary sensory neurons.' Brain Res. 2002, 926, 126-136
3. Nelson, P.J., et al. 'Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells.' J. Cell. Sci. 1999, 112, 361-370
2. Siegel, S.M., et al. 'SSeCKS immunolabeling in rat primary sensory neurons.' Brain Res. 2002, 926, 126-136
3. Nelson, P.J., et al. 'Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells.' J. Cell. Sci. 1999, 112, 361-370